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Hemoglobin H inclusion bodies in RBCs stained with new methylene blue

Hemoglobin H inclusion bodies in RBCs stained with new methylene blue
Red cells from a patient with acquired Hb H disease were incubated in vitro with new methylene blue. Multiple "golf ball-like" inclusions are seen due to precipitation of Hb H. These inclusions form when beta globin tetramers are oxidized by the dye and precipitate.
Hb H: hemoglobin H (beta globin tetramers); MB: methylene blue.
Courtesy of German Pihan, MD, Pathology Department, Beth Israel Deaconess Medical Center, Boston.
Graphic 77536 Version 3.0

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