The scrotal layers from external to internal include the skin, dartos, intercrural fascia, cremaster muscle, infundibulum, parietal tunica vaginalis, and visceral tunica vaginalis. Superficial lacerations of the scrotum (A) should undergo local wound care and closure with absorbable sutures. Penetrating scrotal trauma to or through the dartos layer (B) has a high rate of associated testicular injury and warrants prompt referral to a urologist for surgical exploration in the operating room.