Mohs surgery of squamous cell carcinoma

(A) The excised tissue is subdivided, sequentially numbered, and inked.
(B) A Mohs map of the excised tissue with anatomic landmarks, location of reference nicks, and subdivision and inking.
(C) The excised Mohs tissue is inverted, the angled skin edge is flattened and oriented such that the deep margin and skin edges are aligned in a horizontal plane, embedded in OCT©, and placed on a cryostat chuck.
(D) Tissue is sectioned in a cryostat into thin frozen sections.
(E) Mohs frozen sections stained with hematoxylin and eosin are examined and residual tumor is marked on the Mohs map.