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Eumycetoma etiologic agents: Macroscopic and microscopic features

Eumycetoma etiologic agents: Macroscopic and microscopic features
Organism Macroscopic features and morphology on Sabouraud dextrose agar (SDA) medium Pigment production Microscopic features
Scedosporium boydii Colonies growing rapidly, cottony to woolly, initially dirty white, becoming pale smokey brown, reverse olivaceous black. No pigment Erect synnemata may be present consisting of erect, dark, densely parallel hyphae, producing broadly clavate to cylindrical, subhyaline or pale brown conidia, 6 to 12 × 3.5 to 4.0 mcm.
Madurella mycetomatis Colonies variable, with moderate growth, woolly, yellow or brown. Brownish diffusible pigment Colonies are mostly sterile, composed of a dense, melanized mycelium. Phialides with minute conidia in short chains are rarely present. Sclerotia frequently formed.
Madurella pseudomycetomatis Colonies slowly expanding, dry, brown to grey; reverse brown to grey. Orange-brown pigment Budding cells absent. Hyphae septate, rather thick-walled pale brown. Conidiophores absent. Chlamydospores abundant.
Madurella tropicana Colonies slowly expanding, dry, white-brown to grey; reverse white-brown to grey. Orange-brown pigment Hyphae are septate, thick-walled, pale brown. Conidiophores and conidia are absent or scant conidia alongside hyphae or on inconspicuous phialides. Many chlamydospores are present.
Madurella fahalii Colonies moderately expanding, dry, yellowish brown to grey. Reverse yellowish brown to grey. No pigment secreted Hyphae are septate, thick-walled, pale brown. Conidia produced reluctantly alongside undifferentiated hyphae. Conidia hyaline, smooth-walled, obovoidal to clavate, about 5 to 7 × 2.5 to 3.0 mcm. Chlamydospores numerous.
Falciformispora senegalensis Woolly, dark olive with grey margin, reverse black. No pigment secreted Conidia absent, ascomata developing after 1 to 2 months of incubation, solitary, black (sub)spherical, 100 to 300 mcm diameter. Ascospores subhyaline, obovoidal to ellipsoidal, 23 to 36 × 8.0 to 13.5 mcm usually 4-septate, constricted at septa, with thick, slimy sheath.
Falciformispora tompkinsii Woolly, dark olivaceous-grey, reverse blackish. No pigment secreted Conidia absent, ascomata developing after 1 to 2 months of incubation, solitary, black (sub)spherical, 220 to 530 mcm diameter. Ascospores hyaline, fusiform, 32 to 45 × 9 to 11 mcm usually 6 to 7-septate, slightly constricted at septa, with thick, slimy sheath.
Medicopsis romeroi Colonies flat, evenly velvety or floccose, greyish-sepia to fuscous-black; reverse olivaceous-black. No pigment secreted Pycnidia submerged, ostiolate, black, spherical to pyriform, 85 to 100 mcm in diameter with thick walls. Often covered with stiff, dark hyphae. Conidia produced from ampulliform phialides lining the innermost pycnidial wall, oozing out in slimy drops, hyaline, ellipsoidal to bacilliform.
Trematosphaeria grisea Greenish grey, becoming faint towards the margin; reverse dark brown to black. No pigment secreted Hyphae branched, septate, hyaline or brown, thick-walled, verruculose.
Nigrograna mackinnonii Colonies grey, velvety, becoming dark grey to black with age. No pigment secreted Pycnidia solitary, spherical to pyriform, with papillate ostioles. Conidiogenous cells hyaline, phialidic, discrete. Conidia subhyaline, brown in mass, one-celled, ellipsoidal.
Reference:
  1. de Hoog GS, Guarro J, Gené J, et al. Atlas of Clinical Fungi, 4th ed, Foundation Atlas of Clinical Fungi 2020.
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