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Increase vigilance against cyberattacks
doi : 10.1038/s41587-022-01446-4
Volume 40 Issue 8, August 2022
mRNA printers kick-start personalized medicines for all
Cormac SheridanÂÂ
Drug pipeline 2Q22 � a downturn in approvals
Laura DeFrancescoÂÂ
The digital phenotype of vaccination
Giorgio Quer, Eric J. Topol & Steven R. SteinhublÂ
The BioInnovation Institute as a catalyst for European life science startup creation
Jens Nielsen, Markus J. HerrgÃ¥rd & Bobby SoniÂ
Patents benefit patients and patent reform would spur diagnostic and therapeutic development
Laura A. CoruzziÂÂ
CRISPR comes a-knock-in to reprogram antibodies in vivo
Harald Hartweger & Michel C. NussenzweigÂ
Expanding the spectrum of cancer targets by predicting individual gene fusions
Spatial charting of single-cell transcriptomes in tissues
Runmin Wei, Siyuan He, Shanshan Bai, Emi Sei, Min Hu, Alastair Thompson, Ken Chen, Savitri Krishnamurthy & Nicholas E. NavinÂ
doi : 10.1038/s41587-022-01233-1
Single-cell RNA sequencing methods can profile the transcriptomes of single cells but cannot preserve spatial information. Conversely, spatial transcriptomics assays can profile spatial regions in tissue sections, but do not have single-cell resolution.
Integrative spatial analysis of cell morphologies and transcriptional states with MUSE
Feng Bao, Yue Deng, Sen Wan, Susan Q. Shen, Bo Wang, Qionghai Dai, Steven J. Altschuler & Lani F. WuÂ
m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome
Lulu Hu, Shun Liu, Yong Peng, Ruiqi Ge, Rui Su, Chamara Senevirathne, Bryan T. Harada, Qing Dai, Jiangbo Wei, Lisheng Zhang, Ziyang Hao, Liangzhi Luo, Huanyu Wang, Yuru Wang, Minkui Luo, Mengjie Chen, Jianjun Chen & Chuan HeÂ
doi : 10.1038/s41587-022-01243-z
Functional studies of the RNA N6-methyladenosine (m6A) modification have been limited by an inability to map individual m6A-modified sites in whole transcriptomes. To enable such studies, here, we introduce m6A-selective allyl chemical labeling and sequencing (m6A-SAC-seq), a method for quantitative, whole-transcriptome mapping of m6A at single-nucleotide resolution.
Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro
Bingjie Zhang, Avi Srivastava, Eleni Mimitou, Tim Stuart, Ivan Raimondi, Yuhan Hao, Peter Smibert & Rahul SatijaÂ
doi : 10.1038/s41587-022-01250-0
Technologies that profile chromatin modifications at single-cell resolution offer enormous promise for functional genomic characterization, but the sparsity of the measurements and integrating multiple binding maps represent substantial challenges.
Deep Visual Proteomics defines single-cell identity and heterogeneity
Andreas Mund, Fabian Coscia, András Kriston, Réka Hollandi, Ferenc Kovács, Andreas-David Brunner, Ede Migh, Lisa Schweizer, Alberto Santos, Michael Bzorek, Soraya Naimy, Lise Mette Rahbek-Gjerdrum, Beatrice Dyring-Andersen, Jutta Bulkescher, Claudia Lukas, Mark Adam Eckert, Ernst Lengyel, Christian Gnann, Emma Lundberg, Peter Horvath & Matthias MannÂ
doi : 10.1038/s41587-022-01302-5
Despite the availabilty of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance measurements.
In vivo engineered B cells secrete high titers of broadly neutralizing anti-HIV antibodies in mice
Alessio D. Nahmad, Cicera R. Lazzarotto, Natalie Zelikson, Talia Kustin, Mary Tenuta, Deli Huang, Inbal Reuveni, Daniel Nataf, Yuval Raviv, Miriam Horovitz-Fried, Iris Dotan, Yaron Carmi, Rina Rosin-Arbesfeld, David Nemazee, James E. Voss, Adi Stern, Shengdar Q. Tsai & Adi BarzelÂ
doi : 10.1038/s41587-022-01328-9
Transplantation of B cells engineered ex vivo to secrete broadly neutralizing antibodies (bNAbs) has shown efficacy in disease models. However, clinical translation of this approach would require specialized medical centers, technically demanding protocols and major histocompatibility complex compatibility of donor cells and recipients.
Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells
Pritha Agarwalla, Edikan A. Ogunnaike, Sarah Ahn, Kristen A. Froehlich, Anton Jansson, Frances S. Ligler, Gianpietro Dotti & Yevgeny BrudnoÂ
doi : 10.1038/s41587-022-01245-x
Despite their clinical success, chimeric antigen receptor (CAR)-T cell therapies for B cell malignancies are limited by lengthy, costly and labor-intensive ex vivo manufacturing procedures that might lead to cell products with heterogeneous composition.
A programmable encapsulation system improves delivery of therapeutic bacteria in mice
Tetsuhiro Harimoto, Jaeseung Hahn, Yu-Yu Chen, Jongwon Im, Joanna Zhang, Nicholas Hou, Fangda Li, Courtney Coker, Kelsey Gray, Nicole Harr, Sreyan Chowdhury, Kelly Pu, Clare Nimura, Nicholas Arpaia, Kam W. Leong & Tal DaninoÂ
doi : 10.1038/s41587-022-01244-y
Living bacteria therapies have been proposed as an alternative approach to treating a broad array of cancers. In this study,
Efficient discovery of SARS-CoV-2-neutralizing antibodies via B cell receptor sequencing and ligand blocking
Andrea R. Shiakolas, Kevin J. Kramer, Nicole V. Johnson, Steven C. Wall, Naveenchandra Suryadevara, Daniel Wrapp, Sivakumar Periasamy, Kelsey A. Pilewski, Nagarajan Raju, Rachel Nargi, Rachel E. Sutton, Lauren M. Walker, Ian Setliff, James E. Crowe Jr, Alexander Bukreyev, Robert H. Carnahan, Jason S. McLellan & Ivelin S. GeorgievÂ
doi : 10.1038/s41587-022-01232-2
Although several monoclonal antibodies (mAbs) targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been approved for coronavirus disease 2019 (COVID-19) therapy, development was generally inefficient, with lead generation often requiring the production and testing of numerous antibody candidates.
Accurate detection of tumor-specific gene fusions reveals strongly immunogenic personal neo-antigens
David Weber, Jonas Ibn-Salem, Patrick Sorn, Martin Suchan, Christoph Holtsträter, Urs Lahrmann, Isabel Vogler, Kathrin Schmoldt, Franziska Lang, Barbara Schrörs, Martin Löwer & Ugur SahinÂ
doi : 10.1038/s41587-022-01247-9
Cancer-associated gene fusions are a potential source for highly immunogenic neoantigens, but the lack of computational tools for accurate, sensitive identification of personal gene fusions has limited their targeting in personalized cancer immunotherapy.
Fludarabine increases nuclease-free AAV- and CRISPR/Cas9-mediated homologous recombination in mice
Shinnosuke Tsuji, Calvin J. Stephens, Giulia Bortolussi, Feijie Zhang, Gabriele Baj, Hagoon Jang, Gustavo de Alencastro, Andrés F. Muro, Katja Pekrun & Mark A. KayÂ
doi : 10.1038/s41587-022-01240-2
Homologous recombination (HR)-based gene therapy using adeno-associated viruses (AAV-HR) without nucleases has several advantages over classic gene therapy, especially the potential for permanent transgene expression.
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding
Kerstin U. Ludwig, Ricarda M. Schmithausen, David Li, Max L. Jacobs, Ronja Hollstein, Katja Blumenstock, Jana Liebing, MikoÅ‚aj SÅ‚abicki, Amir Ben-Shmuel, Ofir Israeli, Shay Weiss, Thomas S. Ebert, Nir Paran, Wibke Rüdiger, Gero Wilbring, David Feldman, Bärbel Lippke, Nina Ishorst, Lara M. Hochfeld, Eva C. Beins, Ines H. Kaltheuner, Maximilian Schmitz, Aliona Wöhler, Manuel Döhla, Esther Sib, Marius Jentzsch, Eva-Maria C. Moench, Jacob D. Borrajo, Jonathan Strecker, Julia Reinhardt, Brian Cleary, Matthias Geyer, Michael Hölzel, Rhiannon Macrae, Markus M. Nöthen, Per Hoffmann, Martin Exner, Aviv Regev, Feng Zhang & Jonathan L. Schmid-BurgkÂ
Publisher Correction: Integrative spatial analysis of cell morphologies and transcriptional states with MUSE
Feng Bao, Yue Deng, Sen Wan, Susan Q. Shen, Bo Wang, Qionghai Dai, Steven J. Altschuler & Lani F. WuÂ
Can hackathons unlock a new talent pool from the developing world?
Timothy P. Jenkins, Nicolas Lopez Carranza, Amy Bray, Karim Beguir & Andreas H. LaustsenÂ
